### NGB00362.1:1-61 Illumina Paired End PCR Primer 2.0
perl -p -i -e 's/AGATCGGAAGAGCGGT.*//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAAGAGCGG$//' z-trim-test.trim
### NGB00361.1:1-92 Illumina PCR Primer
perl -p -i -e 's/AGATCGGAAGAGCGTC.*//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAAGAGCGT$//' z-trim-test.trim
### Common region for Illumina PCR Primer and Paired End PCR Primer 2.0
perl -p -i -e 's/AGATCGGAAGAGCG$//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAAGAGC$//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAAGAG$//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAAGA$//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAAG$//' z-trim-test.trim
perl -p -i -e 's/AGATCGGAA$//' z-trim-test.trim
perl -p -i -e 's/AGATCGGA$//' z-trim-test.trim
Removing of homopolymer tails:
perl -p -i -e 's/^A{8,}//' z-trim-test.trim
perl -p -i -e 's/^T{8,}//' z-trim-test.trim
perl -p -i -e 's/^C{8,}//' z-trim-test.trim
perl -p -i -e 's/^G{8,}//' z-trim-test.trim
perl -p -i -e 's/A{8,}$//' z-trim-test.trim
perl -p -i -e 's/T{8,}$//' z-trim-test.trim
perl -p -i -e 's/G{8,}$//' z-trim-test.trim
perl -p -i -e 's/C{8,}$//' z-trim-test.trim
(bio)-informatics, data processing and visualization
Tuesday, September 28, 2010
Illumina adaptor trimming
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment