BLAST-N Plus run parameters and tab-delimited output

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makeblastdb -in Genome_DNA.Fasta -out Genome_DNA.bdb -dbtype nucl -input_type fasta -max_file_sz 2GB -hash_index -parse_seqids 

makeblastdb \

    -in Genome_DNA.Fasta \

    -out Genome_DNA.bdb \

    -dbtype nucl \

    -input_type fasta \

    -max_file_sz 2GB \

    -hash_index \

    -parse_seqids

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blastn -task blastn -query Query_Seqs.Fasta -db Genome_DNA.bdb -out Query_Seqs.vs.Genome_DNA.blastplus.out.m0 -outfmt 0 -evalue 1e-120 -dbsize 1000000 -dust no -word_size 24 -xdrop_ungap 50 -xdrop_gap 500 -xdrop_gap_final 1000 -max_target_seqs 240 -line_length 100 -num_threads 6

blastn \

    -task blastn \

    -query Query_Seqs.Fasta \

    -db Genome_DNA.bdb \

    -out Query_Seqs.vs.Genome_DNA.blastplus.out.m0 \

    -outfmt 0 \

    -evalue 1e-120 \

    -dbsize 1000000 \

    -dust no \

    -word_size 24 \

    -xdrop_ungap 50 \

    -xdrop_gap 500 \

    -xdrop_gap_final 1000 \

    -max_target_seqs 240 \

    -line_length 100 \

    -num_threads 6

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blastn -task blastn -query Query_Seqs.Fasta -db Genome_DNA.bdb -out Query_Seqs.vs.Genome_DNA.blastplus.out.m7 -outfmt '7 qseqid sseqid evalue pident score length nident mismatch gaps frames qstart qend sstart send qcovhsp qlen slen' -evalue 1e-120 -dbsize 1000000 -dust no -word_size 24 -xdrop_ungap 50 -xdrop_gap 500 -xdrop_gap_final 1000 -max_target_seqs 240 -num_threads 6 

blastn \

    -task blastn \

    -query Query_Seqs.Fasta \

    -db Genome_DNA.bdb \

    -out Query_Seqs.vs.Genome_DNA.blastplus.out.m7 \

    -outfmt '7 qseqid sseqid evalue pident score length nident mismatch gaps frames qstart qend sstart send qcovhsp qlen slen' \

    -evalue 1e-120 \

    -dbsize 1000000 \

    -dust no \

    -word_size 24 \

    -xdrop_ungap 50 \

    -xdrop_gap 500 \

    -xdrop_gap_final 1000 \

    -max_target_seqs 240 \

    -num_threads 6   

# Fields: 

query id - 1 [0] (qseqid)

subject id - 2 [1] (sseqid)

evalue - 3 [2] (evalue)

% identity - 4 [3] (pident)

score - 5 [4] (score)

alignment length - 6 [5] (length)

identical - 7 [6] (nident)

mismatches - 8 [7] (mismatch)

gaps - 9 [8] (gaps)

query/sbjct frames - 10 [9] (frames)

q. start - 11 [10] (qstart)

q. end - 12 [11] (qend)

s. start - 13 [12] (sstart)

s. end - 14 [13] (send)

% query coverage per hsp - 15 [14] (qcovhsp)

query length - 16 [15] (qlen)

subject length - 17 [16] (slen)

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