Choosing the right parameters - LAST: find & align related regions of sequences

LAST, our open source implementation of adaptive seeds, enables fast and sensitive comparison of large sequences with arbitrarily nonuniform composition.

https://genome.cshlp.org/content/21/3/487.long

https://gitlab.com/mcfrith/last 

https://gitlab.com/mcfrith/last/-/blob/main/doc/last-cookbook.rst 

Example outputs using various parameters comparing lettuce ribosomal NOR unit 

lastdb -w1 -W1 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta

lastal -k1 -l1  -m10 -j4 -g1.0 -u2 -w0  -D100000 -s2 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.A 

lastal -k1 -l1  -m10 -j4 -g1.0 -u2 -w0    -D1000 -s2 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.B 

 

lastal -k1 -l1  -m10 -j4 -g1.0 -u2 -w0     -D100 -s2 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.C 

 

lastal -k1 -l1 -m100 -j4 -g1.0 -u2 -w0     -D100 -s2 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.D 

 

lastal -k1 -l1 -m100 -j4 -g1.0 -u2 -w0 -W1 -D100 -s2 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.E 

 

lastal -k1 -l1 -m100 -j4 -g1.0 -u0 -w0 -W1 -D100 -s2 LST_01_Ribo_12K.LastDB LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.F 

 

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lastdb -c -w1 -W1 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta

lastal -k1 -l1  -m10 -j4 -g1.0 -u2 -w0  -D100000 -s2 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.x.A 

lastal -k1 -l1  -m10 -j4 -g1.0 -u2 -w0    -D1000 -s2 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.x.B 

lastal -k1 -l1  -m10 -j4 -g1.0 -u2 -w0     -D100 -s2 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.x.C 

lastal -k1 -l1 -m100 -j4 -g1.0 -u2 -w0     -D100 -s2 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.x.D 

lastal -k1 -l1 -m100 -j4 -g1.0 -u2 -w0 -W1 -D100 -s2 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.x.E 

lastal -k1 -l1 -m100 -j4 -g1.0 -u0 -w0 -W1 -D100 -s2 LST_01_Ribo_12K.LastDB.x LST_01_Ribo_12K.Fasta > LST_01_Ribo_12K.vs.Self.TEST.x.F 

 

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>LST_01 Lsat_Tandem_01 m64069_200209_192322.967
CGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGCTGCCACGATCCACTGAGATTCAGCCCTGCGTCGCTCAGATTCGTCCCTCCCCCCCAAAACAAGCCCCCTCATTTTTCCTTCCATGCATACGGACGAGAGGCTGGCTCCCCGACACTTGGTAAAATTTCAGACATTTTGTGACTTGGCGAAAAAAAAGTTCCAAGTCAACCTAAAAAGTTGCCCTTGTCGTATAATGAGTGATGATAGGCCATGGGGGACTACCACCACTTGGTGCCCAGAAGCATATAATGAGTGGACAAGGCATGGTGTTGGGAATTATGCATCCTCGGGGAAATCAGTGTCTGTTCCTGTCACCAAGCTCTTTATGCAATATGTATATATAGGGGGTACATGGGGACTAATACTACCCTTGGTGCCCGACGAGTGTGTTGGGAAACCTAAGCAAGCGAGGCTGGCAAGGCAGGCTACCCAAGGGAACAAGGCACCTGGCCACACACATGCCCATGAACGGCCAAGGGAACAAGGCACCTTGCCACACACACGCCCATGAACTCCCAAGGGAACGAGGCCCCTTGCCACACACATGCCCATCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACATGCCCATCAATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACTTGCCCATCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACTTGCCCATGAACGGCCAAGGAAACAAGGCACCTTGCCACACAGCATGCCCATGAACGACCAAGGGACCAAGGCACCTTGCCACACACATGCCCATCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACTTGCCCATGAACGGCCAAGGAAGCTAGGCCCCTTGCCACACAGCATGCCCCATGAACGACCAAGGGAACGAGGCACCTTGCCACACACATGCCCATCCATGAACGACCAAGGGAAGAATGCATGTGAGGCTGGCAAGGCTAGGTCATGGCAAGCCACACAGTCAGGATACCTATGGGAACAAGGCATCTTGCCACACACATGCCCATGAACGACCAAGGGAACAAGGCACCTTGCCACACACATGCCCATGAACGACCAAGGAAACGAGGCACCTTGCCACACAGCATGCCCATGAACGACCAAGGGAACGAGGCCCCTTGCCACACACATGCCCATCCATGAACGACCAAGGGAACGAGGCCCCTTGCCACACACAGCCCCATGAATGACCAAGGAAGCTAGGCCCCTTGCCACACACATGCCCATCCATGAACGACCAATGGAACGAGGCCCCTTGCCACACAACATGCCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACATGGCCATCCATGAACGACCAAGGGAACATGGCTACTTCCCACACACAGCCCCATGAACGGCCAAGGGAACAGGACTTCTTCCCAAACAGACACCCATGAACGACCAAGTGAACAAGGCTTGCTCCCACACACAGCCCCATGAACGACTAAGGGAACAAGCCTACTTCCTACACAAACACTCATGAACGACCATGAAAACGAGGCATCTTGCCACACACATGCCCTTGAACGAACCAATCCCATCCTCATCGTTCTAAGGAACAAGGGGCCTTGACAAACCATGAGCAGATTTCTAAGCAAGTCAAACGATGAAGGGAGCAAAGTGTTGTAACCGAATCCGTTTAAGTGTTGTAGTTCCTACTTACTACATAGCCACCAGGCGAACTGCTCGTGCTCTTTCGGGTTCTTTCGTTTTGCGACTAATGAATAGCTAGAAGCCTTATCTGCCTACCTATCAGTAGGTGTAGGCGACAGAGACAAAAACCCGAACACGATATATTTCAATTTCAAGGTCTATGTTCACAATGACCCACGCAAAGTTTCAATGACATTCCAACTTGATTTGAGCTGCATATCAACAAGTAGGGAACCGAACGCTTCAAGGCATGGACAGGGATAGGTCATGGACATTTATGCCCCAAACATGACATTTTTTTTATTTCAACGCCTTTCATTTATAATAAAAAGCATCCCTACAAAATTTCGTGGGAATCCGAATTAATTCGAGCGAGTTATGGACGATTTCGCAAAATTATGCATCCCTGGGCAAATCAATGTCTGTTCCTGTCACCAAGCTCTTTGTGCAATATGTATATATAGGGGGTACCAGGGGACTGCTCTCCATCGGCGCCCTGGGGGGTGTCTAGCGCCCAAGGCTGTCGAGGCTGGCACGCTCGAGGCCATGGCCAAGGCGCCCGGTCTTCACGAAAACGAGGCCATCAACGCCCCCATAGACGCCCCACTCGCGTGTCCCCTCGCCCCGACGTCGTGCGTGTCCAAAAATTATGCATCATCAGGGAAATCCATGTCCGTTCCTGTCACCAAGCTCTTTGTGCAATATGTATATATAGGGGGGTACCATGGGGGTCTCATTCGCATGGGTGCCCGACTTGGGCGATGGGCAGCTCAGAGTCATGAGGCTGTATCGAGCACCAACAAGGCAAGCCAAGCCAAGCCCCACGACCCATGAAAGAGGCCAACACCCCCAGCACGCTGCCTTGATTTCTCGAACGATGGCCTGAGAAATAGCCCCGTTGCCTTGACTTTCCTCGACGCCGTGCGCATGAACTAGCCCCGTTGCCTTGATTTTCCTCGACGCCGTGCGCACAAACTAGCCCCGTTGCCTTGATTTTCCTCGACGCCGTGCGCATATTAAAAATTATGCATAATCAGGGAAATCCATGTCTGTTCCTGTCACCAAGCTCTTTGTGCAATATGTATATATAGGGGGGGAGCCGTGAGTGAGCACGGCAAGGGGTGAACGCGCCAGATGGCCTTTCAGCGCGATCATGGGTGACGGTTGCTTAGTTCCGAGTTGCTTAGATAATACCCCTCCGAGTGGGGGCCTTGGCGGGGTGTGGGGATGCCTCGTCAGGTCGCTGCGACAACAGTCCAGGGTTGTGGTCTAGCCTTGGAAATGTGGGATGAGCTGTCTGTGTGGCAATATGATGACGATGTGTGCTTGCTAATCTTGTTCGACGTGCTTCTGGTGCTAGCTAGCATTTGATAATGTGCCGATGATGGGGAAGTGATAGGCGTTAAAGTTGCATGTGTTGCTTTTTGTGATACTACTACGGTACGCTGGTCTATCCTTGTTAGACGTGCGGTTGGGTGCGTAAGAGCTGCCATTGGTTAAGCACCGATAACGTGGAGGACGAGCACTATCGGGAAGCATTGTCAGACATTCAGGATCATCACGGCGTGTTGAAGTTATCTTGGAAGCACAAAAGATGCCAAGCCTGGCTAGCGTCTTTGTGTGGGCGGGTAGCTTCGTACACTGCATCAACCCAAGACTTGCCTTCTCTGAGGTACGATTGGTGCCCATGCCCAGCTAGTGCTGGTCGTACAGGATCAGAGATAGGCATTAAGAGGTCCCTTTTTGCTATCCCCGGCCCAAGCACATACTACATTGCCCATGCCCAGCTAGCAATGATGTGCTTAGGTCAGCAGCGTCGCCTGTCCCTTGTTGCGCATCGTTTGGTGCATTCTGGGGGTTGTTTAAGCTTGTGGTTGCTTGCATGGCCTTGTGCCAAGTGGGTGGCTGTGAGTTTGATGATCTTCGGGGATGTCTACCCTAAAGGTGCATGAGTGGTGTTTGGTTTGTAACGGGTGGTTGGATGTCTGCTTGAGCAGCAACTTCCATGCGTTCTTACCTCTTCAGTTGTGTTACAAGGCGAATTTGCCTTGAACATTGTGGGTTCCTGTGTTGCATACCTAATTGATGGCATTATGCTGTTTCAACAAAGTTTGCTTTCGTTAAGCATCGCTTGCGGTGCCTACGAACCTTGAAGCTGTCTTTGTGGTCCATGTTGTCAATGCGGATGTGTCGATGGCATGGCCTATGAAGTGTTGCTTGGTCTCTTGGATATGGAAGCTGGTGTGGGCACGTGGTCAGTCATGATCATGTATTTTGCCCTACATGAGCGTTTCGCTTCTCTAGACGACTGTCTACCTTGCATTGACTTGTTGGTGCAGGGTAGACTGAGTCGAAGAGGAATGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACAAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTATCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCCGACTTCTGGAAGGGATGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTGCGATGATTCATGATAACTCGACGGATCGCACGGCCCTCGTGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACTATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCTTTCGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGTTGGGTCGGCCGGTCCGCCTTCAGGTGTGCACCGGTTTACTCGTCCCTTCTGTCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATCATAGGATTTCGGTCCTATTACGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTTTAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGTGGAGGTATCCCTCCACGGCCAGCTTCTTAGAGGGACTATGGCCTTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATATAGCCTTGGCCGACAGGCCCGGGAAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATTCCTAGTAAGCGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGGTCCGGTGAAGTGTTAGGATCGCGGCGACGTGGGCGGTTCGCCGCCGGCGACGTCGCGAGAATTCCACTGAACCTTATCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTGTCGAACCCTGCAAGGCAGAACGACCCGTGAACATGTAACCACAACGGGGTGACCGTGATAAGGGCCTCGGTCCTTATCCCCTAACCCTTCCCGACGTGAGTTCGTGGTGTCTTTTTTGGGGCATCATGGATTCCGTTGGACCATAACAAAACCCCGGCACGGTATGTGCCAAGGAAAACAAAAATGAGAAGGACACTACCTGTTTCGCCCCGTTTGCGGTGTGCGTACAGGTCGTGGCCTCCTTGGAATCACAAACGACTCTCGGCAACGGATATCTCGGCTCACGCATCGATGAAGAACGTAGCAAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAACCATCGAGTTTTTGAACGCAAGTTGCGCCCGAAGCCATCCGGCTGAGGGCACGCCTGCCTGGGCGTCACGCATCGCGTCGCTCCCCACCATACCTCCCCAACGGGTTGGCATGGTGTTGGGGGCGGATAATGGCCTCCCGTGCTTGTGTTTCGGTTGGCCTAAATAAGAGTTCCCTTCGGCGGACACACGACTAGTGGTGGTTGAATAGACCCTCGTCTTTTGTTGCGTGTCGTGAGCTGTAAGGGTAGCCCTCATCAAAGACCCCATTGTATCGTCTTCGGATGATGCTTCGACCGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGATCAGCCCAGCTTGAAAATCGGGCGGCCTCGCTGTCCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGGCGGACCGGGCCCAAGTCCCCTGGAAGGGGGCGCCAGAGAGGGTGAGAGCCCCGTCGTGCCCGGACCCTGTCGCACCACGAGGCGCTGTCTGCGAGTCGGGTTGTTTGGGAATGCAGCCCCAATAGGGCGGTAAATTCCGTCCAAGGCTAAATACCGGCGTGAGACCGATAGCAAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGAATGGGGGCCGGCGATGCGTCCCGGTCGGATGTGGAACGGGCGTAAGCCGGTCTGCCGATCGACTCGGGGCGTGGACCGGTGCGGATTGGTGCGGCGGCCAAAGCCCGGACTGTTGATAGGCCCGTGGAGATGCCGTCGCGTCGATCGTGGTTGGCAGCGCGCGCCGTCACGGCGTGCCTCGGCACCTGCGCGCTCCCGGCACCGGCCTGCGGGCACCCCATTCGGCCCGTCTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGCAAGGCGTAAGGAAGCTGATTGGCGGGATCCCCCTAGCGGGGTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCGGGTGCGAGTTCTATCGGGTTAAAGCGAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGTGCGGCTGCTTTGTTGAGCCGTACCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCAAAACTACGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGAAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGCTTAAGCGCGTGACCTACACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGGAGGGCGCGGCGGTCGCTGCAAAACCTTGGGCGTGAGCCTGGGCGGAGCGGCCGTCGGTGCGGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGACGGGGGAAGCCCGTCAGATAGCGCGTTTCGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAACAGCCTGCCCACCCTGGAAACGACTCAGTCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACCGCACGTCGCGCGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGGAGGACCGAGTGCCTCCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCAGTCCCGAACCCGTCGGCTGTTGGCGGACTGCTCGAGCTGCTTCCGCGGCGGAGAGCGGGTCGCTGCGTGCCGGCCGGGGGGACGGACTGGGAACGGCTCCTTCGGGGGCCTTCCCCGGGCGTCGAACAGCCAACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGGATGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTGTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAGTATAAGTGGGAGCCTTCGGGCGAAAGTGAAATACCACTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCAATGCCCCTCTTTTTGGACCCAAGGCCTGCTTCGGCGGGCCGATCCGGGCGGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAGAAGGGTAAAAGCTCGTTTGATTCTGATTTTCCAGTACGAATACGAACCGTGAAAGCGTGGCCTAACGATCCTTTAGACCTTCGGAATTTGAAGCTAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTGGATTGTTCACCCACCAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGCGCTGGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGAAGCGACGCGTGTGCCCGCCGCCTGTTTGCCGACCAGCAGTAGGGGCCTCGGCCCCCAAAGGCACGTGTCGTTGGCTAAGCCTGTGCGACGGATGAGTCGTGCAGGCCGCCATGAAGTATAATTCCCATCAAGCGGCGGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGCTGCCACGATCCACTGAGATTCAGCCCTGCGTCGCTCAGATTCGTCCCTCCCCCCCAAAACAAGCCCCCTCATTTTTCCTTCCATGCATACGGACGAGAGGCTGGCTCCCCGACACTTGGTAAAATTTCAGACATTTTGTGACTTGGCGAAAAAAAAGTTCCAAGTCAACCTAAAAAGTTGCCCTTGTCGTATAATGAGTGATGATAGGCCATGGGGGACTACCACCACTTGGTGCCCAGAAGCATATAATGAGTGGACAAGGCATGGTGTTGGGAATTATGCATCCTCGGGGAAATCAGTGTCTGTTCCTGTCACCAAGCTCTTTATGCAATATGTATATATAGGGGGTACATGGGGACTAATACTACCCTTGGTGCCCGACGAGTGTGTTGGGAAACCTAAGCAAGCGAGGCTGGCAAGGCAGGCTACCCAAGGGAACAAGGCACCTGGCCACACACATGCCCATGAACGGCCAAGGGAACAAGGCACCTTGCCACACACACGCCCATGAACTCCCAAGGGAACGAGGCCCCTTGCCACACACATGCCCATCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACATGCCCATCAATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACTTGCCCATCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACTTGCCCATGAACGGCCAAGGAAACAAGGCACCTTGCCACACAGCATGCCCATGAACGACCAAGGGACCAAGGCACCTTGCCACACACATGCCCATCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACTTGCCCATGAACGGCCAAGGAAGCTAGGCCCCTTGCCACACAGCATGCCCATGAACGACCAAGGGAACGAGGCACCTTGCCACACACATGCCCATCCATGAACGACCAAGGGAAGAATGCATGTGAGGCTGGCAAGGCTAGGTCATGGCAAGCCACACAGTCAGGATACCTATGGGAACAAGGCATCTTGCCACACACATGCCCATGAACGACCAAGGGAACAAGGCACCTTGCCACACACATGCCCATGAACGACCAAGGAAACGAGGCACCTTGCCACACAGCATGCCCAATGAACGACCAAGGGAACGAGGCCCCTTGCCACACACATGCCCATCCATGAACGACCAAGGGAACGAGGCCCCTTGCCACACACAGCCCCATGAATGACCAAGGAAGCTAGGCCCCTTGCCACACACATGCCCATCCATGAACGACCAATGGAACGAGGCCCCTTGCCACACAACATGCCCATGAACGACCAAGGAAGCTAGGCCCCTTGCCACACACATGGCCATCCATGAACGACCAAGGGAACATGGCTACTTCCCACACACAGCCCCATGAACGGCCAAGGGAACAGGACTTCTTCCCAAACAGACACCCATGAACGACCAAGTGAACAAGGCTTGCTCCCACACACAGCCCCATGAACGACTAAGGGAACAAGCCTACTTCCTACACAAACACTCATGAACGACCATGAAAACGAGGCATCTTGCCACACACATGCCCTTGAACGAACCAATCCCATCCTCATCGTTCTAAGGAACAAGGGGCCTTGACAAACCATGAGCAGATTTCTAAGCAAGTCAAACGATGAAGGGAGCAAAGTGTTGTAACCGAATCCGTTTAAGTGTTGTAGTTCCTACTTACTACATAGCCACCAGGCGAACTGCTCGTGCTCTTTCGGGTTCTTTCGTTTTGCGACTAATGAATAGCTAGAAGCCTTATCTGCCTACCTATCAGTAGGTGTAGGCGACAGAGACAAAAACCCCGAACACGATATATTTCAATTTCAAGGTCTATGTTCACAATGACCCACGCAAAGTTTCAATGACATTCCAACTTGATTTGAGCTGCATATCAACAAGTAGGGAACCGAACGCTTCAAGGCATGGACAGGGATAGGTCATGGACATTTATGCCCCAAACATGACATTTTTTTTATTTCAACGCCTTTCATTTATAATAAAAAGCATCCCTACAAAATTTCGTGGGAATCCGAATTAATTCGAGCGAGTTATGGACGA